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KMID : 0385520100230010068
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Analytical Science & Technology
2010 Volume.23 No. 1 p.68 ~ p.73
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Determination of cimetidine injection by square wave voltammetry
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Lee Soo-Jung
Hahn Young-Hee
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Abstract
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In order to develop the square wave voltammetric method determining cimetidine in an ampoule for injection, 5.00×10?4 M cimetidine·HCl solutions prepared with phosphate buffers of various pH values (3.01~8.97) were investigated by SWV. The well defined single peak due to the electrochemical reduction of -C=N-C≡N- in the structure of cimetidine moved towards the cathodic direction by -0.051V/pH as the pH values were increased indicating the involvement of hydrogen in its reduction. The calibration curves of cimetidine·HCl in the concentration range between 1.00×10?5 M and 5.00×10?3 M prepared using three phosphate buffers yielded the slopes of 127,407nA/M (pH 3.01), 115,125nA/M (pH 5.00) and 111,287nA/M(pH 7.00) with excellent linearities of R²?0.9997. When one ampoule of Tagma Inj.® was analyzed by standard addition method by SWV, the within-day precision study (n=4) on the day of sample preparation resulted in the contents of cimetidine as 203±3.8 ㎎ (102% of the specified contents, RSD of 1.9%) and the inter-day precision (n=4) through 5 days was reasonable as 1.3% of RSD.
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KEYWORD
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cimetidine·HCl, square wave voltammetry, electrochemical reduction of -C=N-C≡N-
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